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Probe 2 represents probe 59 in c.
KU Annual Report 2011 – Page 40
Because the efficiency of Southern transfer is lower for larger DNA fragments, upward-shifted bands give an underestimate of the number of extra copies at 1. High molecular weight DNA for pulsed-field gel electrophoresis also was extracted from spleen as follows.
Interestingly, a duplication genotype was observed and there are two plausible explanations for this.
An 8-bp motif should occur approximately once every 64 kb in a random sequence so it seems unlikely to be a coincidence that it should be placed so prominently next to each boundary of the triplicated region. A probe containing this minisatellite detected 13 genomic Blp I fragments at low stringency but only this single locus at high stringency data not shownindicating the presence of similar, but not identical, sequences elsewhere in the mouse genome.
There are several types of genomic rearrangement that could give rise to an increase in copy number, e. To confirm the presence of two extra repeats, the number of extra copies was estimated by PhosphorImager quantification of signal from genomic Southern blots.
Are you looking for the solution to your computer problem? An kb Triplication in Wld s Mice. The composite locus order is centromere – Tnfr2, Nppa – 0.
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Join our site today to ask your question. Unfortunately, it is not clear how to manipulate Wallerian degeneration because little is known about its regulation.
Third, the coding region of the gene may be brought under the action of a new regulatory element by the DNA junctions formed in this rearrangement Fig. Quantification of Hybridization Signals. The mutation occurred spontaneously at Harlan-Olac Bicester, U. Minisatellites have been shown to be highly recombinogenic 26 and involved in genomic rearrangements leading to cancer Early events in the Wallerian degeneration pathway, usually occurring within 24 hr of a lesion, include disintegration of the axonal cytoskeleton and breakdown of the axonal membrane.
Triple6Jul 2, A BAC and P1 contig estimated at 1. Received Apr Unique and Sophisticated Technical Competence. No, create an account now.
Visual inspection of this autoradiograph kk that the shifted band is indeed more intense than the ju band, suggesting that there is more than one extra copy.
The Wld s mutation originally was thought to act by slowing macrophage infiltration into a damaged nerve, but a series of transplantation experiments has shown since that the phenotype is inherent in the axon 89.
There were no discrepancies between the two assays. Either there are a few surviving copies of the proposed original mutation, or partial reversion has lu triplication back to duplication.
It will be interesting to determine whether there is any phenotypic difference between mice carrying the duplication and the triplication, although so far the duplication has been observed only in postmortem tissue.
KU Annual Report – Page 40
Edited by Liane B. Therefore, the most likely interpretation is that the kb shifted band represents a duplication allele and that WldC is either a heterozygote for the kb and kb genotypes or ky somatic mosaic. What model of DELL do you have?
This unusual mutation appears to have been generated by a mechanism involving nonhomologous recombination at a minisatellite and instability in the copy number of the repeat unit. Unfortunately there was insufficient DNA available from WldD to analyze the copy number in this mouse. Sequences at the Boundaries of the Repeat Unit.
Posthybridization washes were normally done at a stringency of 0.